A Fast and Comprehensive Guide RNA Design Tool for Genome Editing, Repression and Activation.

The bacterial antiviral system CRISPR (clustered regularly interspaced short palindromic repeats) has been developed as a flexible technology for multiple purposes of targeted genome engineering, including editing (modifying the genome sequence), gene repression (down-regulation) or activation (up-regulation of the target gene). The system is highly programmable, utilizing an invariant protein , - a nuclease Cas9 for editing or a nuclease-deficient dCas9 or dCas9 fusion protein for repression/activation, - and a custom-designed single guide RNA (sgRNA) for target DNA recognition (Ref). For the commonly used Streptococcus pyogenes CRISPR, target specificity is determined jointly by a 20-nt DNA-binding sequence on the sgRNA and a PAM sequence (protospacer adjacent motif, N(A/G)G) on the DNA.

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