We provide the source code for the generation of
whole-genome sgRNA libraries in other species, useful for genome-wide screening based on
CRISPR, CRISPRi or CRISPRa. Genome sequence and the annotation file from UCSC/NCBI are
needed. Computational environment with bioinformatics software Bowtie et.al. is also
Please download and fill in the Material Transfer Form below, then send it to liuhl3000(at)163.com. We will reply in 3 work days.
Note: CRISPR-ERA genome wide sgRNA design source code is free for academic, nonprofit, and personal use.