A Fast and Comprehensive Guide RNA Design Tool for Genome Editing, Repression and Activation.

We provide the source code for the generation of whole-genome sgRNA libraries in other species, useful for genome-wide screening based on CRISPR, CRISPRi or CRISPRa. Genome sequence and the annotation file from UCSC/NCBI are needed. Computational environment with bioinformatics software Bowtie et.al. is also necessary.

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Note: CRISPR-ERA genome wide sgRNA design source code is free for academic, nonprofit, and personal use.

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