A Fast and Comprehensive Guide RNA Design Tool for Genome Editing, Repression and Activation.

We provide the source code for the generation of whole-genome sgRNA libraries in other species, useful for genome-wide screening based on CRISPR, CRISPRi or CRISPRa. Genome sequence and the annotation file from UCSC/NCBI are needed. Computational environment with bioinformatics software Bowtie et.al. is also necessary.

Please download and fill in the Material Transfer Form below, then send it to liuhl3000(at)163.com. We will reply in 3 work days.

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Note: CRISPR-ERA genome wide sgRNA design source code is free for academic, nonprofit, and personal use.

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